Page 51 - CIBER-BBN2016-ENG

Most relevant scientific articles
• Ojeda E., Puras G., Agirre M., Zarate J., Grijalvo S., Eritja R. et al. The influence of the polar head- group of synthetic cationic lipids on the transfection efficiency mediated by niosomes in rat retina and brain. Biomaterials. 2016;77:267-279.
• Alagia A., Eritja R. siRNA and RNAi optimization. Wiley Interdisciplinary Reviews: RNA. 2016.
• Vengut-Climent E., Gómez-Pinto I., Lucas R., Penalver P., Avinó A., Fonseca Guerra C. et al. Glucose-Nucleobase Pseudo Base Pairs: Biomolecular Interactions within DNA. Angewandte Chemie - International Edition. 2016.
• Hernández-Ainsa S., Ricci M., Hilton L., Avinó A., Eritja R., Keyser U.F. Controlling the reversible assembly of liposomes through a multistimuli responsive anchored DNA. Nano Letters. 2016;16(7):4462-4466.
• Grijalvo S., Mayr J., Eritja R., Díaz D. D. Biodegradable liposome-encapsulated hydrogels for biomedical applications: A marriage of convenience. Biomaterials Science. 2016;4(4):555-574.
Highlights
A number of formulations for the delivery of nucleic acids that have been effective for gene therapy have been successfully developed as a result of an intramural project with the NANOBIOCEL and NN-UMH groups of CIBER-BBN. New formulations have been optimized for the transfection of siRNA molecules in order to facilitate the treatment of degenerative diseases of the retina. To achieve this objective funding was provided through a collaborative project with the companies Sylentis, Leitat and Leadartis. In collaboration with a group from the University of Cambridge lipid-oligonucleotides containing a photosensitive group was developed. The introduction of these compounds into liposomes has allowed the association and dissociation of liposomes by light. It also highlights the success achieved in the design of structures that stabilize non-canonical forms of DNA such as triplex, i-motif and non-natural mating with carbohydrates and its application in the detection of miRNA. The influence of chemical modifications at the ends of the siRNAs on the potency and selectivity of these siRNAs has been studied in detail, obtaining derivatives
with greater potency and greater selectivity in the specific downregulation of the gene expression. Finally, DNA nanostructures of increasing complexity have been prepared in order to facilitate the cellular entry of therapeutic nucleic acids. Early results indicate that the prepared structures facilitate the cellular entry of DNA-based drugs. In order to achieve this objective, there has been an extraordinary funding through an Explora project.
BBN
Research Groups 51